Journal articles: 'Urine humaine' – Grafiati (2024)

APA, Harvard, Vancouver, ISO, and other styles

APA, Harvard, Vancouver, ISO, and other styles

Abstract:

The popularity of phytoestrogen food supplements of plant origin steadily increases. They are used to solve the problems related to the climacterium. Their efficiency depends on woman's capability to metabolise the active components – isoflavonoids. Women able to convert daidzein to S-equol are supposed to get a greater benefit from these supplements. To determine S-equol urine levels, the competitive ELISA was developed. Carboxymethylequol coupled to BSA via 4'-O-position was used as an immunogen. The assay conditions, including the concentrations of the coating antigen and antisera, were optimised. The detection limit was 0.1 ng/ml (5 pg/well) and 50% intercept was 1 ng/ml (50 pg/well). The intraassay coefficients of variation (CV) varied from 4.7% to 9.9%, for the interassay CV the corresponding values of 2.6–11.6% were obtained. The recovery of the standard added to urine ranged between 89% and 104%. All examined samples were obtained from women in climacterium. The volunteers had been taking phytoestrogen food supplements for 3 months. The urine samples were collected before and after the therapy. The levels of free urinary S-equol after the therapy ranged between 0 ng/ml and 42.4 ng/ml. The equol production was observed in 57% of the examined patients. The laboratory results were confronted with patients' subjective evaluations.

APA, Harvard, Vancouver, ISO, and other styles

APA, Harvard, Vancouver, ISO, and other styles

Abstract:

In this study a simple, rapid and sensitive spectrophotometric method was developed for the determination of an antihistaminic drug chlorpheniramine maleate (CPM) in pure form, pharmaceutical preparations, spiked humane urine and spiked blood serum. This method was based on the formation of ion-pairs between the basic nitrogen of the CPM drug and four chromogenic reagents namely bromocresol purple (BCP), alizarine Red S (ARS), eriochrome cyanine R (ECR), and cresol red (CR). The extracted colored ion-pairs were measured spectrophotometrically at 390, 425, 503 and 408 nm for BCP, ARS, ECR and CR reagents, respectively. The different parameters that affect the color development between CPM drug and dyestuff reagents were extensively studied to determine the optimal conditions for the assay procedure. The reaction was studied as a function of the volume of reagents, nature of solvent, temperature, reaction time and stoichiometric ratio between the CPM drug and the reagents. Beer’s law was valid over the concentration ranges of 1-30, 1-10, 2-120 and 4-120 μg mL-1 of CPM drug using BCP, ARS, ECR and CR reagents, respectively. The Sandell sensitivity, molar absorptivity, limit of detection and limit of quantification were determined. Applications of the proposed procedure to the analysis of the pharmaceutical preparations, spiked humane urine and spiked blood serum gave reproducible and accurate results without any interference from excipients. The results obtained by the proposed method were in good agreement with those obtained by reported method. The method can be suggested for the routine analysis of the cited drug.

APA, Harvard, Vancouver, ISO, and other styles

Abstract:

In this study a simple, rapid and sensitive spectrophotometric method was developed for the determination of an antihistaminic drug chlorpheniramine maleate (CPM) in pure form, pharmaceutical preparations, spiked humane urine and spiked blood serum. This method was based on the formation of ion-pairs between the basic nitrogen of the CPM drug and four chromogenic reagents namely bromocresol purple (BCP), alizarine Red S (ARS), eriochrome cyanine R (ECR), and cresol red (CR). The extracted colored ion-pairs were measured spectrophotometrically at 390, 425, 503 and 408 nm for BCP, ARS, ECR and CR reagents, respectively. The different parameters that affect the color development between CPM drug and dyestuff reagents were extensively studied to determine the optimal conditions for the assay procedure. The reaction was studied as a function of the volume of reagents, nature of solvent, temperature, reaction time and stoichiometric ratio between the CPM drug and the reagents. Beer’s law was valid over the concentration ranges of 1-30, 1-10, 2-120 and 4-120 μg mL-1 of CPM drug using BCP, ARS, ECR and CR reagents, respectively. The Sandell sensitivity, molar absorptivity, limit of detection and limit of quantification were determined. Applications of the proposed procedure to the analysis of the pharmaceutical preparations, spiked humane urine and spiked blood serum gave reproducible and accurate results without any interference from excipients. The results obtained by the proposed method were in good agreement with those obtained by reported method. The method can be suggested for the routine analysis of the cited drug.

APA, Harvard, Vancouver, ISO, and other styles

Abstract:

Hormonanalysen im Urin werden vorwiegend angewendet, um Störungen des Steroidhormonmetabolismus, um eine erhöhte Produktion biogener Amine und um nicht invasiv humanes β-Choriogonadotropin als Proteinhormon als Indikator einer Schwangerschaft nachweisen zu können. Diese Untersuchungen müssen gegebenenfalls von Plasma-/ Serumbestimmungen komplementiert werden. Klinische Daten einschließlich der aktuellen Medikation sowie das Urinkreatinin als Bezugsgröße sind unabdingbar zur seriösen Beurteilung einer Urinuntersuchung. Die wichtigsten Krankheitsbilder, die mittels Steroidhormonanalyse untersucht werden können, sind der Exzess eines typischen oder atypischen mineralokortikoid wirksamen Steroidhormons, die Hormonaktivität eines Nebennieren- oder ovariellen Tumors, Akne unklarer Ätiologie, Hirsutismus, ein PCO-, ein adrenogenitales oder ein vermutetes Cushingsyndrom. Die Untersuchung biogener Amine wird durchgeführt bei Verdacht auf eine sekundäre bzw. therapierefraktäre Hypertonie, bei Auftreten phäochromozytom- oder paragangliom-assoziierter Symptome oder Verdacht auf einen serotininproduzierenden Tumor. Bei Kindern steht die Suche nach genetisch determinierten Erkrankungen im Vordergrund.

APA, Harvard, Vancouver, ISO, and other styles

Abstract:

SUMMARYEscherichia coliO157.H7 was found in 10 of 3570 (0·28%) faecal samples from dairy cattle in 5 of 60 herds (8·3%). Several tentative associations with manure handling and feeding management practices on dairy farms were identified. Faecal/urine slurry samples, bulk milk samples, and milk filters from dairy herds were negative forE. coli0157.H7.E. coli0157.H7 was also isolated from 10 of 1412 (0·71 %) faecal samples from pastured beef cattle in 4 of 25 (16%) herds. The prevalence ofE. coli0157. H7 excretion in feedlot beef cattle was 2 of 600 (0·33 %). The identification of cattle management practices associated with colonization of cattle byE. coli0157.H7 suggests the possibility that humanE. coliO157.H7 exposure may be reduced by cattle management procedures.

APA, Harvard, Vancouver, ISO, and other styles

APA, Harvard, Vancouver, ISO, and other styles

APA, Harvard, Vancouver, ISO, and other styles

Abstract:

ABSTRACT. Two simple precise and accurate spectrophotometric techniques are developed for simultaneous determination of ternary mixture of urea, creatinine, and uric acid in humane urine sample. The first technique is a ratio derivative zero - crossing where measured urea at 216.61 nm, creatinine at 260.56 nm, and uric acid at 283 nm. The second technique is a successive derivative of ratio spectra where urea, creatinine, and uric acid measured at 203 nm, 261 nm, and 283 nm, 287 nm, respectively. These procedures do not need separation. The proposed methods showed excellent linear range over the concentration ranges of 1.0–15.0 μg/mL, 1.0–16.0 μg/mL, and 2.0–15.0 μg/mL for urea, creatinine, and uric acid, respectively. The recoveries ranged from 97.10% to 101.9% for urea, 97.22% to102.70 % for creatinine, and 97.45% to102.55 % for uric acid with relative standard deviations less than 1.56% for urea, 3.87% for creatinine, and 3.71% for uric acid. The analytical eco-scale and green analytical procedure index tools were used to evaluate how the proposed procedures will affect the environment. The simultaneous quantification of urea, creatinine, and uric acid in human urine samples can be accomplished with remarkable effectiveness by using the suggested approaches. KEY WORDS: Urea, Creatinine, Uric acid, Derivative spectrophotometry Bull. Chem. Soc. Ethiop. 2023, 37(4), 817-829. DOI: https://dx.doi.org/10.4314/bcse.v37i4.2

APA, Harvard, Vancouver, ISO, and other styles

Abstract:

Une approche holistique des questions environnementales met en lumière les limites atteintes par l’utilisation des égouts pour éloigner les excrétats humains des espaces habités. Les mégapoles, en particulier, sont aujourd’hui exposées à des enjeux aigus et encore méconnus. Dans un contexte de changements globaux majeurs auxquels l’assainissem*nt doit faire face, une si grande concentration de population met le modèle classique de l’assainissem*nt sous tension. À la saturation récurrente des systèmes d’assainissem*nt centralisés, s’ajoute une empreinte élevée en matière de dépense énergétique, d’émissions de gaz à effet de serre et souvent de pollution des milieux aquatiques. Pourtant, ces excrétats, gérés séparément, pourraient être utilisés comme matières fertilisantes : leur utilisation agricole permet de sortir d’une logique de dépollution des excrétats et de créer un mutualisme entre territoires urbains et agricoles, ces derniers bénéficiant alors de matières fertilisantes pérennes, locales et non fossiles. À partir du cas de l’agglomération parisienne, nous proposons une analyse transversale des opportunités actuelles de mise en oeuvre de filières de séparation à la source en contexte de grande concentration urbaine. Plus spécifiquement, nous nous intéressons aux freins et leviers techniques, organisationnels, économiques, en vue de la production de fertilisants à partir des excrétats humains et en particulier des urines, dans de bonnes conditions sanitaires et agronomiques. Nous montrons l’accumulation d’éléments de démonstration quant aux limites du système d’assainissem*nt actuel et la nécessité d’un changement de paradigme. Persiste, nonobstant, une difficulté à mettre en place des projets pilotes (freins politiques, socioculturels, économiques, réglementaires et techniques, exacerbés dans le contexte d’une mégapole). Toutefois, une dynamique récente s’observe, avec des projets qui émergent en marge de la fabrique urbaine. Un facteur clé de leur réussite tient à leur adaptation aux différentes configurations territoriales. Portés par des individus et collectifs aux trajectoires spécifiques, ceux-ci laissent entrevoir une capacité à inventer de nouveaux dispositifs sociotechniques de gestion des excrétats humains.

APA, Harvard, Vancouver, ISO, and other styles

Abstract:

L'étude comparative de deux tombes crématoires de la Vénétie (à Padoue et à Este), chacune datant de la fin du sixième siècle av. J-C, nous permet de mieux comprendre les étapes du rituel mortuaire. La complexité des découvertes archéologiques ne peut s'expliquer qu'au moyen d'une définition tout aussi complexe du système mortuaire, en tenant compte d'éléments tels que: le nombre d'individus à l'intérieur d'une tombe, les modifications apportées à un cercueil ou à une urne cinéraire, le mélange des objets présents dans la tombe et des os incinérés de plusieurs individus à l'intérieur de la même urne etc. Afin de constituer un organigramme, on a pris en compte à la fois les analyses micro-stratigrafiques, la soigneuse restauration des objets découverts, l'analyse de vestiges humains ainsi que des reconstructions expérimentales. Ce travail vise à acquérir une meilleure compréhension des codes/pratiques funéraires dans les sociétés complexes de l'Italie pendant l'Âge du Fer.

APA, Harvard, Vancouver, ISO, and other styles

APA, Harvard, Vancouver, ISO, and other styles

Abstract:

In clinical and pathanatomic practice, doctors face a very heterogeneous group of human and animal diseases caused by a violation of the reduction of the urinary duct during embryogenesis. The pathology of urachus is a complex problem of humane and veterinary medicine. We conducted a thorough clinical study of foals who had pathology in the navel area. In case of death, the foal was opened and the organs of the urinary system, the umbilical vein and arteries were examined in detail. It was found that such foals did not have obliteration of the primary urinary duct (urachus) during the embryonic period and its flattening because of separation from the abdominal wall during labor. As a result, urine from the bladder got into the subcutaneous tissue, and then, after the umbilical cord stump fell off, it began to stand out through the umbilical opening. Complications of this pathological condition were edema, uremia, bleeding into the bladder, which led to respiratory arrest.

APA, Harvard, Vancouver, ISO, and other styles

APA, Harvard, Vancouver, ISO, and other styles

Abstract:

AbstractTraining using positive reinforcement is increasingly recognised as a valuable tool for the humane and effective management and use of laboratory-housed non-human primates. We utilised a mixed-mode questionnaire to survey use of training and other learning processes (socialisation, habituation and desensitisation) in over half of UK establishments using and breeding primates. The survey demonstrated that there is widespread awareness of training as a refinement technique and appreciation of its diverse benefits, but training is not used as widely or as fully as it might be. This is due to real constraints (principally staff and time and a lack of confidence in ability to train), and perceived constraints (such as a supposed lack of published information on how to train and assessment of the benefits, and an overestimation of the time investment needed). There is also considerable variation between establishments in the purposes of training and techniques used, with a reliance on negative reinforcement in some. We conclude that there is opportunity for refinement of common scientific, veterinary and husbandry procedures (such as blood and urine collection, injection, capture from the group and weighing) through use of positive reinforcement training, especially when combined with appropriate socialisation, habituation and desensitisation. We end this paper with recommendations on best practice, training techniques and staff education.

APA, Harvard, Vancouver, ISO, and other styles

Abstract:

Penelitian ini bertujuan untuk mendapatkan data lokasi, etiologi, hasil kultur kuman dan uji sensitifitas antibiotik serta komplikasi dari penderita abses leher dalam yang di rawat ruang rawat inap bedah teratai THT-KL RSUD Dr. Soetomo periode Januari sampai Desember 2014. Bahan dan metode penelitian yaitu rekam medik penderita. Hasil: Didapatkan 41 penderita. Lokasi di submandibula 13(31,70%), ruang peritonsil 11(26,82%), kombinasi ruang submandibula, parafaring dan retrofaring 11 (26,82%). Etiologi infeksi gigi 14(34,14%), infeksi tonsil akut 8(19,51%), DM 8(19,51%), DM Tipe 2 dan infeksi gigi 10(24,39%), DM disertai infeksi orofaring 1(2,43%). Dari kultur pus didapatkan Staph. Aureus (33,33%), Strep. viridians (28,75%), Kleb. Pneumonia (9,52%). Dari kultur darah didapatkan kuman Strep. viridians (40,00%), Kleb. Pneumonia (20,00%), Acinobacter humanii (20,00%), Staph. Haemolyticus (20,00%). Kultur urin didapatkan Acinobacter humanii dan Staph. haemolyticus masing-masing 50%. Hasil uji sensitifitas antibiotik dari kultur pus didapatkan angka sensitive terhadap Meropenem (87,5%), Ceftriaxone (73,68%), Cefoperazone-sulbactam (70,00%), Cefotaxime (68,75%). Resitensi kuman terhadap antibiotika tersebut diatas secara berurutan 12,50%, 21,05%, 20,00%, 12,5%. Komplikasi sepsis 12(29,26%), obstruksi jalan napas atas (trakeotomi) 6(14,63%). Kesimpulan: Lokasi tersering di submandibula disusul peritonssil, kombinasi submandibula dan parafaring atau retrofaring. Etiologi tersering infeksi gigi disusul tonsil. Penyakit penyerta tersering yaitu DM. Kuman yang sering ditemukan dari kultur pus adalah Staph. aureus dan Strep. viridians. Kuman dari kultur darah terbanyak Strep. viridians, disusul Kleb. pneumonia, Acinobacter humanii, Staph. haemolyticus. Hasil uji sensitifitas terhadap antibiotik dari kultur pus didapatkan angka sensitif tertinggi adalah Meropenem disusul Ceftriaxone, Cefoperazone-sulbactam dan Cefotaxime. Komplikasi tersering sepsis disusul obstruksi jalan nafas atas dan mediastinitis. Sebanyak 4,87% penderita meninggal.

APA, Harvard, Vancouver, ISO, and other styles

Abstract:

Zusammenfassung. Unter dem Begriff «Porphyrie» wird eine Gruppe von acht Stoffwechselerkrankungen zusammengefasst, welche in Folge einer gestörten Hämbiosynthese entstehen. Die Einteilung der verschiedenen Formen erfolgt anhand der klinischen und biochemischen Merkmale. Dieser Artikel fokussiert auf die akuten (hepatischen) Porphyrien (AHP), die durch eine ähnliche Pathophysiologie und klinische Symptomatik gekennzeichnet sind. Typisch sind akute, das autonome Nervensystem betreffende Symptome; starke, kolikartige, teilweise ausstrahlende Oberbauchschmerzen, Übelkeit, Erbrechen, Hypertonie, Tachykardie, Obstipation und Hyponatriämie. Die Diagnose einer AHP wird aufgrund einer mindestens fünffach erhöhten Ausscheidung von Porphobilinogen (PBG) im Urin gestellt. Therapie der Wahl ist die Zufuhr von Kohlenhydraten, die Gabe von humanem Häm, die Elimination und Vermeidung von schubauslösenden Triggerfaktoren und eine begleitende symptomorientierte Therapie, im Besonderen eine suffiziente Analgesie.

APA, Harvard, Vancouver, ISO, and other styles

Abstract:

Knowledge about African swine fever virus (ASFV) transmission and its survival in the environment is mandatory to develop rational control strategies and combat this serious disease in pigs. In this study, the risk that environmental contamination poses for infection of naïve pigs was investigated. Naïve pigs were introduced as sentinels into contaminated pens kept at ambient temperature (about 18–22˚C) either on the same day or up to 3 days after ASFV-infected pigs were removed. Three experiments were carried out in which four to six pigs per pen were inoculated with virulent ASFV isolates OURT88/1 (genotype I), Georgia 2007/1, or POL/2015/Podlaskie (genotype II), respectively. The majority of the inoculated pigs developed acute disease but with no evident haemorrhagic lesions or haemorrhagic diarrhoea and were culled at the predefined humane endpoint. The levels of ASFV DNA detected in the blood of the infected animals reached 107−9 genome copies/ml before euthanasia. Environmental swabs were taken from different surfaces in the animal rooms, as well as from faeces and urine, close to the time of introduction of the naïve animals. Relatively low quantities of virus DNA were detected in the environmental samples, in the range of 103−7 genome copies per swab or per gram and ml of faeces and urine. No infectious virus was recovered from these environmental samples. Neither clinical signs nor virus genomes were detected in the blood of any of the sentinel pigs over a period of 2 to 3 weeks after exposure, indicating that transmission from the ASFV-contaminated environment did not occur. Interestingly, viral DNA was detected in nasal and oral swabs from some of the sentinel animals at early days of exposure (ranging between 103.7−5.8 genome copies per swab), though none of them developed ASF. The results indicate a relatively low risk of ASFV transmission from a contaminated environment under the conditions provided in these experimental studies and in the absence of bloodshed from infected animals.

APA, Harvard, Vancouver, ISO, and other styles

Abstract:

La présente étude est une revue critique de la documentation scientifique sur les effets du bruit sur la santé mentale. Cette étude a été réalisée sur la base de la recherche de mots-clés, de l’impact factor des revues, du nombre de fois que l’article a été cité. Hinari a servi de base des données. L’analyse et la sélection des articles scientifiques nous ont permis de les regrouper en trois catégories d’études. Les études fondamentales ont dosé les taux des hormones de stress dans le plasma, les urines et la salive. Les études expérimentales ont exposé les sujets humains à la pollution sonore pour évaluer les déficits de leurs mémoire, attention et performance. Les études épidémiologiques ont évalué les déficits cognitifs chez les élèves étudiant dans des milieux bruyants et l’état de santé mentale des individus habitant aux alentours des aéroports, voies ferrées et des autoroutes. Les études fondamentales et expérimentales ont abouti à un consensus scientifique sur les effets négatifs du bruit tandis que les résultats des études épidémiologiques ne permettent pas de tirer des conclusions solides, des études ultérieures s’avèrent donc nécessaires.

APA, Harvard, Vancouver, ISO, and other styles

Abstract:

An 11-year-old spayed female domestic shorthair cat was evaluated for anorexia, lethargy and weight loss of 6 days’ duration. Bilateral mydriasis, absent menace response, slow-to-absent pupillary light reflexes, bilateral retinal detachment, intermittent horizontal nystagmus, intermittent ventral strabismus and systemic hypertension were present. Biochemical analysis revealed severe hyponatremia, severe hypochloremia and mild hypokalemia. Multifocal central nervous system disease was suspected based on optic, trigeminal sensory (ophthalmic branch), vestibulocochlear and possible oculomotor nerve dysfunction. Thoracic radiographs showed mild cardiomegaly without evidence of congestive heart failure. Ultrasound revealed mild pleural and peritoneal effusion. A cause of the severe hyponatremia was not identified, and it persisted despite fluid therapy. Syndrome of inappropriate antidiuretic hormone secretion (SIADH) was suspected as the cause of hyponatremia. Humane euthanasia was elected owing to continued clinical decline. Serum hyposmolality, urine hyperosmolality, natriuresis and lack of confirmed renal, thyroid and pulmonary disease aided in the presumed diagnosis of SIADH. Post-mortem histopathology of the brain revealed degeneration of the hypothalamus and optic tracts, along with a prominent fluid-filled craniopharyngeal duct (putative Rathke’s cleft cyst) separating the pars distalis and the pars intermedius. The hypothalamic degeneration, possibly secondary to a Rathke’s cleft cyst, was hypothesized to be the cause of presumptive SIADH in the patient. Although rare in occurrence, Rathke’s cleft cyst should be included as a differential diagnosis in dogs and cats with signs of pituitary dysfunction.

APA, Harvard, Vancouver, ISO, and other styles

Abstract:

Objective: In the Czech Republic, it is possible, to carry out Medical Termination of Pregnancy (MToP) in the 1st trimester up until the 49th day of secondary amenorrhea. The aim of the study is to analyse the significance of serum/urine human chorionic gonadotropin (hCG) assessment and ultrasound (US) examination in pregnancy diagnosis and MToP follow-up. Methods: In 2017–2018, MToP was carried out in a total of 109 women by administering a combination of mifepristone (600 mg orally) and misoprostol (400 mcg orally). Serum/urine (LSUP – low sensitivity urine pregnancy test) hCG assessment and US examination were performed at pregnancy diagnosis and MToP follow-up. Results: At pregnancy diagnosis, there was a positive and medium strong correlation between serum hCG and size of the gestational sac – GS (R = 0.711; P < 0.0001) and crown-rump length of the embryo – CRL (R = 0.605; P < 0.0001). Gestational age was 42–49 days (average 45.6, median 45 days), the women were 16–44 years of age (average 29.4, median 29 years). In MToP follow-up, serum hCG level was > 1,000 IU/L in 13.8% of women (15/109) and a positive LSUP test in 17.4% (20/109). US examination diagnosed ongoing pregnancy in five women and missed abortion in one woman (serum hCG was always > 1,000 IU/L and LSUP test was always positive). In 5.5% of women (6/109), a subsequent surgical intervention was carried out including those with ongoing pregnancy (N = 5); missed abortion (N = 1) was treated by additional misoprostol, where surgical intervention was not necessary. Conclusion: At pregnancy diagnosis, there is a positive and medium strong correlation between serum hCG and CRL. In MToP follow-up, a negative LSUP test enables reliable exclusion of ongoing pregnancy and missed abortion. In case of a positive LSUP test, US examination should be performed; however, surgical intervention should not be indicated solely on the basis of uterine cavity dilatation. Key words: medical termination of pregnancy – 1st trimester – human chorionic gonadotropin – ultrasound

APA, Harvard, Vancouver, ISO, and other styles

Abstract:

Introdução: A exploração do manganês no Amapá e o armazenamento inadequado dos rejeitos de minério na área portuária de Santana geraram impactos ambientais e sociais aos moradores locais. Objetivo: Avaliar a presença de elementos inorgânicos nas moradoras do bairro Elesbão expostas aos rejeitos de minérios. Método: Por meio da Espectrofotometria de Absorção Atômica de Chama (F-AAS), quantifcaram-se metais na água utilizada pela população e na matriz biológica urina, enquanto no cabelo utilizou-se Espectrometria de Massas com Plasma Indutivamente Acoplado (ICP-MS). Coletou-se o sangue de 67 mulheres expostas e de 99 não expostas, destas, coletou-se 65 amostras de urina e 18 de cabelo. Foram realizadas análises hematológica, bioquímica e de coagulação no sangue de todas as participantes que também responderam a um questionário socioeconômico. Resultados: Quanto à faixa etária, 60,0% das moradoras expostas estavam entre 18 e 42 anos e moravam na localidade entre 10 e 34 anos. Na água analisada, encontrou-se concentrações elevadas dos metais As, Cd, Cu, Fe, Mn e Pb. Na matriz urina, observou-se signifcância para o Mn e, no cabelo, para Pb e Mn. Dentre os parâmetros hematológicos signifcativos estavam Hb, Ht, VCM, HCM, monócitos, alterações morfológicas nas hemácias e plaquetas. Na avaliação bioquímica, observaram-se alterações nas enzimas fosfatase alcalina, bilirrubinas e creatinina. Conclusões: Há contaminação ambiental por elementos que podem comprometer a saúde da população exposta, quando em contato por tempo prolongado. Estudos mais aprofundados e direcionados à saúde da mulher precisam ser realizados, incluindo parâmetros como a análise de hormônios e marcadores genéticos, visando contribuir, assim, para uma ação das autoridades competentes em melhorar a saúde da população exposta.

APA, Harvard, Vancouver, ISO, and other styles

APA, Harvard, Vancouver, ISO, and other styles

Abstract:

ZusammenfassungDie hypophysären Gonadotropine follikelstimulierendes Hormon (FSH) und luteinisierendes Hormon (LH) spielen eine zentrale Rolle bei der Steuerung der Gonadenfunktionen. Daher ist ihr Einsatz in der Therapie von Fruchtbarkeitsstörungen (z. B. Azyklie) sowie in der Biotechnologie (z. B. Superovulation, Hormonprogramme zur Zyklussynchronisation) prinzipiell von hohem Interesse. Präparationen von FSH bzw. LH sind aufgrund der aufwendigen Gewinnung aus Hypophysengewebe relativ teuer und daher besonderen Anwendungen vorbehalten. Bei Primaten- und Equidenarten wurde die Expression eines LH-ähnlichen Moleküls im Chorionepithel nachgewiesen (Choriongonadotropin, CG). Equines CG (eCG) weist außer bei Equiden, bei denen es ausschließlich an LH-Rezeptoren bindet, bei allen bei uns üblichen Haussäugetierspezies neben seiner LH-Aktivität eine außerordentlich hohe FSH-Aktivität auf („duale Wirkung“). Seit seiner Markteinführung kommt ihm daher eine hohe Bedeutung als vergleichsweise kostengünstiges FSH-Analogon vorwiegend zur Anwendung bei Wiederkäuern und Schwein zu. Im Gegensatz zu dem als LH-Analogon eingesetzten humanen CG (hCG), das nicht invasiv aus dem Urin schwangerer Frauen isoliert werden kann, muss die Gewinnung von eCG aus dem Blut trächtiger Spenderstuten erfolgen, da im Urin nur minimale eCG-Konzentrationen vorliegen. Nach Berichten über Todesfälle und Leiden von Spenderstuten im Zusammenhang mit der eCG-Gewinnung in südamerikanischen Haltungen ist das derzeitige Verfahren der eCG-Produktion zunehmend in die öffentliche Kritik geraten, was zuletzt in Forderungen nach einem generellen Verbot mündete. Ziel dieses Beitrags ist daher, den aktuellen Kenntnisstand zu Eigenschaften und Biologie dieses auch aus Sicht der Grundlagenwissenschaft hochinteressanten Moleküls kurz zusammenzufassen.

APA, Harvard, Vancouver, ISO, and other styles

Abstract:

Цель исследования — изучение ионного обмена, а также водо- и азотовыделительной функции почек у крыс под влиянием фармакологической стимуляции мелатониновых рецепторов. Эксперимент был проведен на крысах-самцах, которые были разделены на две группы: 1-я — контрольная (n=35), содержалась в стандартных условиях вивария; 2-я — на протяжении всей жизни получала мелатонин вместе с питьевой водой (n=35). Исследование проведено с соблюдением международных принципов Хельсинкской декларации о гуманном отношении к животным. По анализу крови и мочи определяли потребление воды, объем диуреза, содержание креатинина, мочевины, ионов натрия и калия в моче и крови. На основании полученных данных рассчитывали параметры водно-солевого обмена. Полученные в результате исследования данные подвергали статистической обработке. В результате эксперимента отмечено снижение функциональных способностей почек у крыс как в контрольной группе, так и в группе животных, получавших мелатонин. Однако анализ данных показывает, что крысы, получавшие мелатонин, имели более высокие показатели водовыделительной, ионорегулирующей и азотовыделительной функций почек по сравнению с интактными животными. Таким образом, длительное применение экзогенного мелатонина приводило к более стабильному функционированию почек и водно-солевого обмена у старых животных в сравнении с контрольной группой. The aim of the study was to study ion exchange, as well as water and nitrogen excretion function of the kidneys in rats under the infl uence of pharmacological stimulation of melatonin receptors. The experiment was conducted on male rats, which were divided into 2 groups. The fi rst group was a control group (n=35) and was kept in standard vivarium conditions. Animals of the second group received melatonin along with drinking water throughout their lives (n=35). The study was conducted in compliance with the international principles of the Helsinki Declaration on Humane Treatment of Animals. According to the blood and urine analysis, water consumption, the volume of diuresis, the content of creatinine, urea, sodium and potassium ions in urine and blood were determined. Based on the data obtained, the parameters of water-salt metabolism were calculated. The data obtained as a result of the study were subjected to statistical processing. As a result of the experiment, a decrease in the functional abilities of the kidneys was noted in rats both in the control group and in the group of animals treated with melatonin. However, data analysis shows that rats treated with melatonin had higher values of indicators of water-releasing, ion-regulating and nitrogen-releasing kidney functions compared with intact animals. Thus, long-term use of exogenous melatonin led to more stable functioning of the kidneys and water-salt metabolism in old animals compared with the control group.

APA, Harvard, Vancouver, ISO, and other styles

Abstract:

IntroductionFew studies have assessed the efficacy of smoking cessation interventions in individuals with type 2 diabetes, but interventions adapted to the specific needs of this population are warranted. The aim of this study is to assess the efficacy of a smoking cessation intervention in a population of smokers with type 2 diabetes and to measure the metabolic impact of smoking cessation.Methods and analysisThe study is an open-label, randomised control trial. Participants recruited from a sanitary region of Switzerland will be randomly allocated to either the intervention or the control arm. The intervention group will have four individual counselling sessions over 12 weeks. Trained research nurses will conduct the behavioural intervention, using motivational interviews and addressing diabetes and gender specificities. The control group will have one short counselling session at baseline and will be given written information on smoking cessation. Both groups will have a follow-up visit at 26 and 52 weeks. Demographic and medical data will be collected at baseline and follow-up, along with blood and urine samples. The primary study outcome is continuous smoking abstinence validated by expired-air carbon monoxide from week 12 to week 52. Secondary study outcomes are continuous and 7-day point prevalence smoking abstinence at 12 and 26 weeks; change in motivation to quit and cigarette consumption; and change in glycosylated haemoglobin levels, body weight, waist circumference and renal function after smoking cessation. In a subsample of 80 participants, change in stool microbiota from baseline will be measured at 3, 8 and 26 weeks after smoking cessation.Ethics and disseminationEthical approval has been obtained by the competent ethics committee (Commission cantonale d’éthique de la recherche sur l’être humain, CER-VD 2017–00812). The results of the study will be disseminated through publications in peer-reviewed journals and conference presentations.Trial registration numbersClinicalTrials.gov NCT03426423 and SNCTP000002762; Pre-results.

APA, Harvard, Vancouver, ISO, and other styles

Abstract:

Mycobacterium arupense is a non-tuberculous, potentially pathogenic species rarely isolated from humans. The aim of the study was to ascertain the spectrum of non-tuberculous mycobacteria within 271 sequenced mycobacterial isolates not belonging to M. tuberculosis and M. avium complexes. Isolates were collected between 2004 and 2009 in the Czech Republic and were examined within the framework of ecological studies carried out in animal populations infected with mycobacteria. A total of thirty-three mycobacterial species were identified. This report describes the isolation of M. arupense from the sputum of three human patients and seven different animal and environmental samples collected in the last six years in the Czech Republic: one isolate from leftover refrigerated organic dog food, two isolates from urine and clay collected from an okapi (Okapia johnstoni) and antelope bongo (Tragelaphus eurycerus) enclosure in a zoological garden, one isolate from the soil in an eagle's nest (Haliaeetus albicilla) band two isolates from two common vole (Microtus arvalis) livers from one cattle farm. All isolates were identified by biochemical tests, morphology and 16S rDNA sequencing. Also, retrospective screening for M. arupense occurrence within the collected isolates is presented.

APA, Harvard, Vancouver, ISO, and other styles

APA, Harvard, Vancouver, ISO, and other styles

Abstract:

Urinary catheterization has risks, and its use should be limited. Instrumentation of the urinary tract is responsible for up to 80% of nosocomial urinary tract infections. Other side effects are common including urinary retention when attempting to remove the catheter. Urinary catheterization is not indicated to obtain a urine sample for culture in hospitalized elderly patients with a suspected urinary tract infection who cannot provide a urine specimen on demand. The risks of catheterization are clear and urinary culture results can lead to inappropriate treatment, whereas the physician’s clinical assessment rather than the culture results are most important in directing antibiotic therapy.

APA, Harvard, Vancouver, ISO, and other styles

Abstract:

Nilai gizi yang banyak pada jagung manis sehingga menyebabkan meningkatnya permintaan pasar cukup tinggi. Penelitian ini bertujuan agar dapat menganalisis interaksi varietas jagung manis yang unggul dan jenis urine hewan yang terbaik. Percobaan ini telah dilakukan di Kelurahan Taba Jemekeh Kecamatan Lubuklinggau Timur I Kota Lubuklinggau dengan elevasi 114 mdpl. Percobaan dilaksanakan dari bulan Maret 2021 sampai bulan Mei 2021. Percobaan ini diterapkan melalui metode Rancangan Acak Kelompok (RAK) faktorial meliputi 2 faktor perlakuan, dengan faktor pertama pengujian 3 taraf perlakuan varietas jagung manis yaitu : V1 = Bonanza, V2 = Talenta, V3 = Sweet Boy dan faktor kedua 3 taraf perlakuan jenis biourine U1 = Sapi, U2 = Kambing, U3 = Kerbau, dan menghasilkan 9 kombinasi setiap unit percobaan dengan 3 kali pengulangan dan terdapat 27 unit uji coba terdiri dari 3 percontoh. Hasil percobaan menunjukkan kombinasi perlakuan antara varietas jagung manis Bonanza F1 dan aplikasi urine sapi (V1U1) memberikan respon pertumbuhan dan produksi terbaik dan pemberian biourine ternak secara tunggal pada parameter tinggi tanaman, jumlah daun, panjang akar, panjang tongkol, berat tongkol per batang dan kadar gula memberi respon sangat nyata.

APA, Harvard, Vancouver, ISO, and other styles

Abstract:

Objectives: To study the frequency of different clinical presentations of kala pathar poisoning at Jinnah Hospital Lahore. Study design: Cross sectional study Place and Duration: Department of Medicine, Jinnah Hospital, Lahore from April 2021 to October 2021. Methodology: A total of 90 patients with h/o kala pathar poisoning of both sex and of age 20-50 year were included. Case sheet of patients were prepared for age & sex and clinical presentation i.e. pain in throat, dysphagia, dysphonia, cervico-facial edema, difficulty in opening mouth, dark urine, acute renal failure, sinus tachycardia and sinus bradycardia. Results: Mean age was 33.69 ± 7.91 years. Out of 90 patients, 54 (60.0%) were males and 36 (40.0%) were females with male to female ratio of 1.5:1. Mean duration of kala pathar intake was 7.33 ± 5.86 hours. The main presentation of kala pathar poisoning was pain in throat (98.89%), dysphagia (100%), dysphonia (100%), cervico-facial edema (97.78%), difficulty in opening mouth (98.89%), dark urine (86.67%), acute renal failure (44.44%), sinus tachycardia (88.89%) and sinus bradycardia (21.11%). Conclusion: This study concluded that pain in throat, dysphagia and dysphonia are the common clinical presentations of kala pathar poisoing followed by cervico-facial edema, difficulty in opening mouth, dark urine, acute renal failure, sinus tachycardia and sinus bradychardia. Keywords: Kala pathar poisoning, dysphagia, dysphonia, angioedema, sinus tachycardia.

APA, Harvard, Vancouver, ISO, and other styles

APA, Harvard, Vancouver, ISO, and other styles

Abstract:

Diabetes mellitus is a very silent disease, which, according to various studies, has been growing every year, among them are patients with type 2 diabetes mellitus that is characterized by why they do not produce enough insulin in their body, causing them to inject insulin in an uncontrolled way, caused discomfort by the number of times they inject. Some patients do not know if they have type 2 diabetes, and over time several inefficient and expensive diabetes screening systems have been developed, limiting their use by everyone. Similarly, if this disease is not detected in time, it can compromise other parts of the body so it is always necessary to control your eating habit, on the other hand, this type 2 disease can be detected in fasting of the patient. According to the problem exposed, in this research a self-detection system of type 2 diabetes mellitus was carried out by aspirating a urinalysis to detect through the sweet smell of urine if the patient may have type 2 diabetes mellitus by means of gas sensors and carbon nanotubes fused by a microcontroller. Through the operation of the system, it was observed that the tests were performed with an efficiency of 98.99%, being an accepted value for a reliable and safe diabetes analysis, demonstrating that it can detect type 2 diabetes mellitus by aspirating the smell of urine.

APA, Harvard, Vancouver, ISO, and other styles

Abstract:

Elevated level of blood glucose hyper glycemia leads to spillage of glucose in t urine, hence that terms sweet urine. Minority populations are disproportionately affected by diabetes. Self care for diabetes is of an utmost importance for prevention. Diabetes mellitus is a group of metabolic diabetes characterized by the elevated levels of glucose in the blood (hyperglycemia) resulting from defects in insulin secretion, insulin action or both. Insulin a hormone produced by the pancreas controls the level 0f glucose in the blood by regulating the production and storage of glucose. HbA1c levels improved more with combined exercise compared with either supervised aerobic or supervised resistance exercise alone. Diabetes self-management is the cornerstone of diabetes control and averting complications. If not treated properly, it can lead to a variety of chronic problems, including irreparable disability and death. Majority 13 (43.3%) of them were secondary, 9 (30%) of them were moderate, 8(26.6%) of them were heavy working pattern.

APA, Harvard, Vancouver, ISO, and other styles

Abstract:

Aim: In this study, it was aimed to determine the frequency of asymptomatic Human Immunodeficiency Virus (HIV), Hepatitis B, Hepatitis C, and syphilis in circumcised patients diagnosed with urethritis transmitted by sexual intercourse because of unprotected sexual contact. Material and Methods: We retrospectively investigated the serological results of HIV, Hepatitis B, Hepatitis C, and Syphilis diseases in 364 male patients diagnosed with urethritis, all of them were circumcised during childhood. The study included patients who applied to the urology outpatient clinic of secondary state hospital between January 2017 and December 2019 with symptoms or signs of urethritis. In the examination, only urethral discharge could be seen without symptoms. After the patients were examined, first void urine samples were taken. Also at the first examination, peripheral blood samples were tested for HIV, Hepatitis B, Hepatitis C, and syphilis antibodies. Results: As a result of retrospective screening of the serological results of 364 male patients diagnosed with urethritis, Hepatitis B positivity was 1.09% with 4 cases, Hepatitis C positivity was found as 0.27% in 1 case and the Syphilis positivity rate was 1.92% with 7 cases in 364 patients. None of the patients had HIV positivity. Conclusions: The fact that there was not any encounter of HIV-positive patients in patients diagnosed with urethritis because of unprotected sexual contact led to the thought that circumcision had a protective contribution in these patients with urethritis, who were all circumcised. Also, screening tests, especially syphilis, should be performed on all patients diagnosed with STI infection.

APA, Harvard, Vancouver, ISO, and other styles

Abstract:

Metabolic syndrome is considered to be a cluster of disorders that directly contribute to the development of cardiovasculardisease and are characterized by chronic systemic infl ammation. Numerous epidemiological data indicate that an adverseintrauterine environment, caused by the peculiarities of the nutritional status or placental insuffi ciency in a woman with metabolic syndrome, can “program” the susceptibility of the fetus to further development of cardiovascular and metabolic diseases, has an impact on cognitive and behavioral development. Nitric oxide (NO) plays a critical role in the pathogenesis of components of the metabolic syndrome. Children born prematurely have a high incidence of brain damage, which can lead to motor, cognitive, behavioral, social and sensory disorders.The purpose the research was the study of the effect of the eNOS gene polymorphism and indicators of nitric oxidemetabolism on the neonatal consequences in prematurely born children from mothers with metabolic syndrome.Material and methods. A study was conducted in which 100 premature infants were included. Two groups were formed: the main group (n=34), which included preterm infants (birth weight 2145.29±148.19 g and gestational age 33.18±0.55 weeks) of mothers with metabolic syndrome, and the comparison group (n=66), which included preterm infants (birth weight 2295.99±101.45 and gestational age 34.03±0.45 weeks) of mothers without metabolic syndrome. The children underwent a genetic study – determination of the polymorphism of the eNOS gene, as well as the level of nitrites, nitrates and nitrosothiols in the urine.By decision of the bioethics commission No. 217 dated 12.06.2023, the materials of the scientifi c work comply with the Rulesof Humane Treatment of Patients.Traditional methods of parametric and nonparametric statistics were used; nonparametric methods were used to analyzequalitative characteristics expressed mainly in percentages. The methods of parametric statistics were used to check the normality of the distribution of quantitative characteristics using the Kolmogorov- Smirnov criterion.Statistical processing of the obtained results was carried out using the package of application programs EXCEL-2003® andSTATA version 11 for Windows (StataCorp, Texas, USA).The work was carried out as part of the scientifi c and experimental work of the Department of Pediatrics #1 with Neonatologyof the Poltava State Medical University “To develop clinical and laboratory criteria, methods of predicting and preventingmetabolic disoders in young children (state registration number 0120U102856).Results. The most common diseases in the infants of the studied groups were the consequences of intrauterine hypoxia(44.1%) and respiratory failure requiring artifi cial lung ventilation (50.0%), although no signifi cant diff erences were foundin the prevalence of these conditions. We identifi ed the presence of signifi cant associations between the consequences ofintrauterine hypoxia and the levels of nitrates (OR 1.19; 95% CI 1.01-1.40; p=0.042), nitrosothiols (OR 1.19; 95% CI 0.99-1.42;p=0.050) and the polymorphic genotype 4aa/ab of the eNOS gene (OR 0.28; 95% CI 0.12-0.67; p=0.004). Analysis of systemichemodynamics revealed no signifi cant diff erences in baseline values between preterm infants with and without intrauterinehypoxia, but we did observe an association with urine output on day 3 of life.To fi nally clarify the complex infl uence of indicators of nitrate metabolism on the development of intrauterine hypoxia andto predict the development of consequences of this condition in premature infants, the following indicators are included in theregression prognostic model: the level of nitrates, nitrites, 4aa/4ab genotype and urine output on the third day of life. As theresearch results show, there is a direct reliable relationship with nitrates and an inverse relationship with nitrites, 4aa/4abgenotype and urine output. This prediction model has high operating characteristics – the area under the ROC curve is 0.8168.Some mechanisms of the infl uence of maternal metabolic syndrome on the development of relevant disorders in newborns areknown, including disorders of nitric oxide synthesis, endothelial dysfunction, and oxidative stress. In our study, the consequences of intrauterine hypoxia were reliably associated with an increase in the concentration of urinary nitrates and a decrease in nitrites, as well as the absence of the 4aa/ab genotype, which is associated with reduced release of nitric oxide. There is evidence that nitric oxide can have both protective and deleterious eff ects, depending on factors such as nitric oxide synthase isoform and duration of exposure to hypoxia.Conclusions. In preterm infants born to mothers with metabolic syndrome, elevated urinary nitrate levels and the absenceof the eNOS 4aa/ab genotype increase the likelihood of suff ering the consequences of intrauterine hypoxia.

APA, Harvard, Vancouver, ISO, and other styles

Abstract:

One of the most common conditions treated in adult primary care medicine is Urinary Tract Infection (UTI), which accounts for a sizeable portion of antibiotic prescriptions. A high degree of diagnostic accuracy is necessary because this issue is so prevalent and important in everyday clinical practice. Particularly in light of the rising prevalence of antibiotic resistance, excessive antibiotic prescriptions should be avoided. To examine the machine learning approach and Internet of Things (IoT) for urinary tract infections, this research proposes an Ensemble Bagging Decision Tree Classifier (EBDTC). In our study, to learn more about UTI, we conducted a study in which we collected the physiological data of 399 patients and preprocessed them using the min-max scalar normalization. Feature extraction using Principle Component Analysis (PCA) and classification using Ensemble Bagging Decision Tree Classifier (EBDTC). The performance outcomes of accuracy (96.25%), precision(96.22%), recall (98.07%), and f-1 measure(97.17%) demonstrate the proposed strategy's significantly improved performance in comparison to other baseline existing techniques.

APA, Harvard, Vancouver, ISO, and other styles

Abstract:

Malignant hyperthermia (MH) is a life-threatening clinical syndrome of hypermetabolism involving the skeletal muscle. It is triggered in susceptible individuals primarily by volatile inhalational anesthetic agents and the muscle relaxant succinylcholine. MH affects humans, certain pig breeds, dogs, horses, and probably other animals. The classic signs of MH include A dramatic rise in body temperature, rigid or painful muscles, especially in the jaw, flushed skin, sweating, an abnormally rapid or irregular heartbeat, rapid breathing or uncomfortable breathing, brown or cola-colored urine, very low blood pressure (shock), confusion and muscle weakness or swelling after the event. The syndrome is likely to be fatal if untreated. This study therefore assessed the effects of sevoflurane gas on the vital signs of Cavia porcellus with malignant hyperthermia. For the determination of malignant hyperthermia, 50, 15 day-old weaned kits of Peru breed were placed in an anesthesia chamber for 5 minutes and subjected to a mixture of air with 3% sevoflurane gas. Only 6% of them reacted positively, while most of them, 94%, were negative. The average body temperature of the positive kits was 38.9ºC, oscillating between 38.7 and 39.1 ° C, while the negative ones averaged 37.9 ° C. The positive kits had an average of 142.3 breaths per minute compared to the negative ones, which was determined to be 83.7. The number of heart beats per minute in the kits with a positive reaction to sevoflurane averaged 205 and the negative ones 119.5. Muscle tremors were observed throughout the body during exposure of the positive kits to sevoflurane gas. It is concluded that for every 16 guinea pigs, 1 will react to the sevoflurane gas exposure positive due to the result obtained from the vital sign. Only the positive ones presented muscle tremors. L'hyperthermie maligne (HM) est un syndrome clinique d'hypermétabolisme mettant en jeu le pronostic vital impliquant le muscle squelettique. Elle est déclenchée chez les individus sensibles principalement par des agents anesthésiques volatils par inhalation et le relaxant musculaire succinylcholine. MH affecte les humains, certaines races de porcs, les chiens, les chevaux et probablement d'autres animaux. Les signes classiques de MH comprennent une augmentation spectaculaire de la température corporelle, des muscles rigides ou douloureux, en particulier dans la mâchoire, une peau rougeoyante, une transpiration, un rythme cardiaque anormalement rapide ou irrégulier, une respiration rapide ou une respiration inconfortable, une urine brune ou de couleur cola, très faible tension artérielle (choc), confusion et faiblesse ou gonflement musculaire après l'événement. Le syndrome estsusceptible d'être fatal s'il n'est pas traité. Cette étude a donc évalué les effets du gaz sévoflurane sur les signes vitaux de Cavia porcellus avec hyperthermie maligne. Pour la détermination de l'hyperthermie maligne, 50 chatons sevrés de 15 jours de race Pérou ont été placés dans une chambre d'anesthésie pendant 5 minutes et soumis à un mélange d'air avec 3% de sévoflurane. Seuls 6 % d'entre eux ont réagi positivement, alors que la plupart d'entre eux, 94 %, ont été négatifs. La température corporelle moyenne des kits positifs était de 38,9 ° C, oscillant entre 38,7 et 39,1 ° C, tandis que les kits négatifs étaient en moyenne de 37,9 ° C. Les kits positifs avaient une moyenne de 142,3 respirations par minute par rapport aux négatifs, ce qui a été déterminé à être 83,7. Le nombre de battements cardiaques par minute dans les kits avec une réaction positive au sévoflurane était en moyenne de 205 et ceux négatifs de 119,5. Des tremblements musculaires ont été observés dans tout le corps pendant l'exposition des kits positifs au sévoflurane. Il est conclu que pour 16 cobayes, 1 réagira positivement à l'exposition au gaz sévoflurane en raison du résultat obtenu à partir du signe vital. Seuls les positifs présentaient des tremblements musculaires.

APA, Harvard, Vancouver, ISO, and other styles

Abstract:

This article presents research on robot therapy for elderly residents in a care house. Experiments were conducted from June 2005, lasting more than 2 months. Two therapeutic baby seal robots were introduced to the residents, and activated for over 9 hours daily. To investigate the psychological and social effects of the robots, the residents’ activities in public areas were recorded using video cameras, during the daytime (8:30–18:00) for over 2 months. In addition, urinalysis of the residents was performed for 17-ketosteroid sulfate and 17-hydroxycorticosteroid. Results of the video analysis indicated that social interaction increased through interaction with the seal robots. Results of the urine tests showed that the reactions of the subjects’ vital organs to stress improved after the introduction of the robots.

APA, Harvard, Vancouver, ISO, and other styles

APA, Harvard, Vancouver, ISO, and other styles

Abstract:

Centrifuge alternatif dari motor kipas angin dengan kebaruan rancangan dan peralatan yang digunakan berupa motor kipas angin, rotor centrifuge yang dirancang dengan kemiringan 30° dan selongsong rotor menggunakan spuit 20 cc. Penelitian dilakukan karena terbatasnya instrumentasi yang menunjang kegiatan pengelolaan laboratorium. Penelitian ini bertujuan membuat inovasi centrifuge alternatif dengan menggunakan motor kipas angin yang bisa diaplikasikan untuk preparasi berbagai sampel dilaboratorium. Penelitian menggunakan metode eksperimen dan analisa data secara deskriptif. Dilakukan perancangan centrifuge menggunakan motor kipas angin, perancangan rotor centrifuge dengan bahan plat besi yang didesain menggunakan mesin CNC. Selanjutnya perakitan, uji coba dan pengujian terhadap beberapa sampel. Uji coba dilakukan terhadap sampel tanah, katalis Ni.CaO dan urin dengan kecepatan putaran 1400 rpm, diamati volume cairan dan padatan hasil preparasi dan Gambaran mikroskopis selanjutnya dilakukan komparasi hasil antara centrifuge alternatif hasil rancangan dengan centrifuge pabrikan. Dari pengujian dan pengamatan, centrifuge alternatif hasil rancangan bisa dipergunakan untuk preparasi sampel tanah, katalis NI.CaO dan urin diperoleh hasil yang hampir sama dengan centrifuge pabrikan dan bisa digunakan untuk kegiatan dilaboratorium.

APA, Harvard, Vancouver, ISO, and other styles

Abstract:

Konsumen rumah tangga semakin selektif dalam memenuhi kebutuhan pangan termasuk kebutuhan sayuran segar. Preferensi konsumen cenderung memilih produk sayuran yang terjamin bebas dari penggunaan pestisida kimia. Kecenderungan tersebut merupakan peluang sekaligus tantangan bagi petani agar memanfaatkan penggunaan pupuk organik dan pestisida nabati sebagai wujud penerapan teknologi pertanian semi organik. Tujuan pengabdian ini supaya petani memiliki pengetahuan teknologi pertanian semi organik dalam upaya menghasilkan produk sayuran segar yang memenuhi persyaratan keamanan pangan. Tujuan dicapai melalui pelaksanaan kegiatan pengabdian kepada masyarakat dengan menggunakan metode pendidikan orang dewasa (andragogy) dan fasilitas pembelajaran demonstrasi plot (demplot) dan tutorial tatap muka, serta pengendalian kualitas produk sayuran segar melalui pengawasan proses produksi. Hasil pengabdian menunjukkan bahwa petani telah memiliki pengetahuan dan kemampuan praktek penerapan teknologi pertanian semi organik pada tanaman bayam cabut dan sawi caisim, namun baru 20 persen dari semua petani yang tergabung dalam Kelompok Tani Embun Pagi telah menerapkan penggunaan pestisida nabati dan POC Urine, serta 100 persen telah menerapkan penggunaan pupuk organik kotoran ayam pedaging atau meningkat 50%.

APA, Harvard, Vancouver, ISO, and other styles

Abstract:

Background: Urinary Tract Infection (UTI) is one of the most common infections in children. Use of antibiotics according to updated sensitivity pattern is critical. Aim: To determine major bacteria causing UTI with drug sensitivity pattern to find best empirical therapy. Study design: Descriptive cross-sectional study. Methodology: This study was conducted at WAPDA hospital Rawalpindi from June 2022 to November 2022. A total of 71 urine culture positive children aged between 1 to 14 years were enrolled. The main variables for the study included age and sex of patient, isolated bacteria and the sensitivity pattern against different drugs. The data was analyzed using SPSS version 23.0. The variables are presented in the form of standard deviation and frequency of percentages. Results: The average age of enrolled children was 5.9 (SD ± 3.6) years. The common bacteria leading to UTI were E. coli 43 (60.6%) followed by Klebsiella 11 (15.5%) and Enterococcus 5 (7%). The most common organism isolated was E. coli. The most effective drugs against E. coli are Meropenem (97.2%), Fosfomycin (90.2%), and Amikacin (89.7%). The resistance pattern is Co-trimoxazole (86%), Amoxicillin/clavulanic acid (69.8%) and Cefixime (70.7%). Conclusion: The most common isolate was E. coli. The sensitivity of nitrofurantoin, Fosfomycin and Amikacin to the pathogens’ causing UTI is high and can be used empirically. Other commonly used antibiotics are mostly resistant, therefore regular studies in every region are necessary to update the protocols for treatment of UTI. Keywords: Urinary tract infection, E. coli, Amikacin, Urine culture and sensitivity

APA, Harvard, Vancouver, ISO, and other styles

Abstract:

An opportunistic investigation into ecosystem instability in Kachemak Bay (KBay), Alaska, has led us to investigate exposure to toxic algae in sea otters. We used gene expression to explore the physiological health of sea otters sampled in KBay in May 2019. We found altered levels of gene transcripts in comparison with reference sea otters from clinically normal, oil-exposed, and nutritionally challenged populations sampled over the past decade. KBay sea otters were markedly divergent from the other groups for five genes, which indicated the involvement of neurological, cardiac, immune, and detoxification systems. Further, analyses of urine and fecal samples detected domoic acid in the KBay sea otters. In combination, these results may point to chronic, low-level exposure to an algal toxin, such as domoic acid. With a warming climate, the frequency and severity of harmful algal blooms in marine environments is anticipated to increase, and novel molecular technologies to detect sublethal or chronic exposure to algal toxins will help provide an early warning of threats to the stability of populations and ecosystems.

APA, Harvard, Vancouver, ISO, and other styles

Abstract:

Kelompok Tani Arsa Winangun merupakan kelompok tani ternak yang telah di bina oleh tim kegiatan pengabdian PHP2D Fakultas Peternakan Universitas Udayana Tahun 2020. Dimana kegiatan yang dilakukan adalah peningkatan pengetahuan kelompok tersebut dalam memanfaatkan sumber daya yang ada seperti feses dan urine untuk dijadikan pupuk organic serta hijauan yang dijadikan silase untuk pakan ternak dengan memanfaakan teknologi Bio-Balitani. Desiminasi teknologi pemanfaatan produk Bio-Balitani yang diproduksi memanfaatkan konsorsium bakteri Lignosellulolitik asal rumen sapi bali dan rayap merupakan salah satu strategi yang dikembangkan untuk mengatasi berbagai permasalahan yang ada di Kelompok Tani Ternak Arsa Winangun. Sosialisasi dan desiminasi teknologi, pembentukan demoplot serta pengembangan strategi pemasaran di Kelompok Tani Arsa Winangun merupakan kegiatan yang dilaksanakan dalam upaya mengatasi kurangnya pengetahuan serta keterampilan kelompok tani dalam pengelolaan limbah peternakan dan pemasaran produk yang dihasilkan. Hasil pelaksanaan kegiatan menunjukkan bahwa Kelompok Tani Ternak Arsa Winangun mampu menyerap teknologi yang didiseminasikan dengan baik yang ditunjukkan dengan adanya kemampuan dari kelompok tersebut dalam produksi pupuk organik dengan memanfaatkan teknologi Bio-Balitani yang siap dipasarkan.

APA, Harvard, Vancouver, ISO, and other styles

APA, Harvard, Vancouver, ISO, and other styles

Abstract:

Objectives: To determine the frequency of acute kidney injury (AKI) in patients with diabetic Ketoacidosis at National Institute of Child Health (NICH). Materials and Methods: Totally 80 patients who were enrolled. Demographics data were noted. Five ml venous blood sample and urine sample were collected from of each child in aseptic conditions in sterilized container and sent to laboratory of the hospital for random blood sugar (RBS), serum urea, serum creatinine, serum electrolytes (Na, K, Ph,) complete blood count (CBC, hemoglobin (Hb), white blood cells (WBC), platelets count, arterial blood gas (ABG), and ketones. All were drawn at the time of admission and after 24 hours. All children were catheterized at time of admission to monitor urine output which is part of DKA protocol and was recorded at 6, 12, 24 hours. AKI were labelled according to KDIGO classification as stage I, II, III depending upon rise in serum creatinine from baseline or according to urine output at 6, 12, 24 hours. All the demographic details and duration of diabetes mellitus and DKA and its treatment were recorded on a predesigned data collection proforma. Results: Out of 80 patients, 44.3% were male and 55.7% were female with mean age of 9.63± 3.34 years. 67% of the patients were aged 7.1-14 years, followed by 27% patients were aged 1-7 years and only 5.7% patients were aged of 14.1-18 years. The mean Duration of hospital stay was 6.88±1.45 days. The Mean Hb and WBCs, and Platelets level were 12.13±1.9 g/l, 16.37±7.97 and 348836.3±140355.4 respectively. The mean PH of all the enrolled patients was found 7.08±0.14 and mean Bicarbonate was 7.54±3.37mmol/L. The mean RBS at admission time and after 24-hour treatment were 403.7±95.42 mg/dl and 266.7±65.1 mg/dl respectively. The mean Serum Urea at admission time and after 24-hour treatment were 36.6±28.9 mg/dl and 36.9±23.5 mg/dl respectively. And the mean serum creatinine (mg/dl) at admission time and after 24-hour treatment were 0.75±0.58 and 0.76±0.55 mg/dl respectively. The mean electrolytes (Na, K, Ph) at admission time and after 24 hours were 138.4±7.0 mmol/l and 139.1±4.0 mmol/l, 4.0±0.83 mg/dl and 4.88±4.83 mg/dl, 19.2±30.7 mg/dl and 16.1±22.0 mg/dl respectively. Frequency of AKI was 31.8% and patients needed for peritoneal dialysis were 3.4%. Practical implication: In our study it was find out that AKI is a common complication in patients with Diabetic ketoacidosis. So this study will help the clinical practioner to also treat the patients for acute kidney injury that will provide early recovery from all the symptom of acute kidney injury. Conclusion: AKI is a common complication in children who are admitted for DKA and clinical practitioner should take precautionary parameter to avoid such complication. Keywords: Acute Kidney Injury, Diabetic Ketoacidosis, Children, DM, DM Type-1, child health, Frequency, NICH

APA, Harvard, Vancouver, ISO, and other styles

Abstract:

Herlyn Werner Wunderlich Syndrome (HWW) is a rare congenital malformation of female urogenital tracts. We report our first case of HWWS with urethrovagin*l fistula in Lady Aitchison Hospital Lahore. A 27 years old lady presented with hematuria and urinary retention having single opening at vulva with menstrual blood and urine draining through it. Examination under anesthesia showed uretheral opening in anterior wall of lower 1/3 of vagin*.MRI showed uterus didelphus with two cervices and vagin* (atresia of lower 2/3 on left side) and absent left kidney.Surginal treatment by creating a separate opening of neourethra was made by Urology Department of Mayo Hospital. Keywords: Herlyn Werner Wunderlich syndrome, urethrovagin*l fistula, urogenital tract

APA, Harvard, Vancouver, ISO, and other styles

Abstract:

Desa Penimbung Kabupaten Lombok Barat berpotensi menjadi desa wisata karena merupakan jalur utama menuju bendungan PLTA Meninting. Salah satu spot wisata yang ingin diwujudkan adalah spot wisata edukasi berbasis pertanian organik dengan melibatkan petani milenial (pemuda tani). Permasalahan yang dihadapi pemuda tani di Desa Penimbung adalah kurangnya pengetahuan dan ketrampilan untuk mengembangkan sistem pertanian organik. Oleh karenanya perlu adanya transfer teknologi untuk meningkatkan pemahaman dan ketrampilan pemuda tani di desa ini. Solusi yang ditawarkan untuk menjawab permasalahan ini adalah membekali pemuda tani dengan pengetahuan dan ketrampilan mengenai sistem pertanian organik, salah satunya dengan pelatihan pembuatan pupuk organic cair (POC) dengan memanfaatkan sumberdaya yang ada di desa. Kegiatan yang ditawarkan adalah berupa penyuluhan dan bimbingan praktek pembuatan pupuk organik cair dari bahan dasar urin sapi plus akar sereh. Kegiatan dilakukan dalam beberapa tahap untuk membentuk pemahaman secara konsepsi dan ketrampilan dalam memanfaatkan limbah peternakan untuk membuat pupuk organik cair. Kegiatan pengabdian ini diikuti oleh 16 orang peserta dari kelompok pemuda tani yang ada di Desa Penimbung. Seluruh rangkaian kegiatan berjalan dengan baik dan peserta antusias mengikuti kegiatan sejak dari penyiapan bahan dan tempat, penyuluhan, hingga mempraktekkan pembuatan pupuk organik cair dari urin sapi plus akar sereh. Dengan adanya bekal teori sekaligus ketrampilan diharapkan kelompok pemuda tani yang menjadi mitra kegiatan pengabdian ini dapat mengimplementasikan ilmu yang telah diperoleh secara mandiri untuk kemajuan Desa Penimbung di masa depan.